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Liquid Chromatography/tandem Mass Spectrometry Method for Simultaneous Determination of Risperidone and Its Active Metabolite 9‐Hydroxyrisperidone in Human Plasma by Jignesh Bhatt; Gunta Subbaiah; Sadhana Singh is a Chemistry article available to read on EtoBox.

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## Abstract A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for simultaneous quantification of risperidone (RSP) and its active metabolite 9‐hydroxyrisperidone (9‐OH‐RSP) in human plasma. The analytes were extracted from human plasma by using the protein precipitation extraction technique. Methyl risperidone was used as internal standard for RSP and 9‐OH‐RSP. A Betasil C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The mass transition ion‐pair was followed as __m/z__ 411.28 → 191.15 for RSP and __m/z__ 427.30 → 207.10 for 9‐OH‐RSP. The method involves a simple extraction, isocratic chromatography conditions and mass spectrometric detection that enable detection at sub‐nanogram levels. The proposed method has been validated with a linear range of 0.10–15.0 ng/mL for RSP and 9‐OH‐RSP. The intrarun and interrun precision and accuracy values were within 15%. The overall recoveries for RSP and 9‐OH‐RSP were 82.1% and 83.2%, respectively. The total analysis time was as low as 3.0 min only. The developed method was applied for the determination of the pharmacok

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Author

Jignesh Bhatt; Gunta Subbaiah; Sadhana Singh

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)