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This research article presents a novel method for heritable genome editing in tomato using a Tobacco rattle virus (TRV)-based system that delivers the ISYmu1 TnpB endonuclease without the need for transgenesis or tissue culture. The study successfully generated virus-free homozygous mutant progeny by targeting the SlPDS gene and demonstrated the potential for functional analysis of the SlDA1 locus, resulting in enlarged fruits. This approach offers a streamlined, efficient, and less genotype-dependent strat

Author

youshust1991

Language

EN