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Quantitative Analysis of Cocaine and Its Metabolites in Whole Blood and Urine by High-performance Liquid Chromatography Coupled with Tandem Mass Spectrometry by Sys Stybe Johansen; Helle Merete Bhatia is a Chemistry article available to read on EtoBox.
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## Aim: In forensic toxicology it is important to have specific and sensitive analysis for quantification of illicit drugs in biological matrices. this paper describes a quantitative method for determination of cocaine and its major metabolites (ecgonine methyl ester, benzoylecgonine, norcocaine and ethylene cocaine) in whole blood and urine by liquid chromatography coupled with tandem mass spectrometry lc/ms/ms. ## Method: The sample pre-treatment (0.20 g) consisted of acid precipitation, followed by centrifugation and solid phase extraction of supernatant using mixed mode sorbent columns (spec mp1 ansys diag. inc.). chromatographic separation was performed at 30 degrees c on a reverse phase zorbax c18 column with a gradient system consisting of formic acid, water and acetonitrile. the analysis was performed by positive electrospray ionisation with a triple quadropole mass spectrometer operating in multiple reaction monitoring (mrm) mode. two mrm transitions of each analyte were established and identification criteria were set up based on the retention time and the ion ratio. the quantification was performed using deuterated internal analytes of cocaine, benzoylecgonine and ecgoni
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It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Sys Stybe Johansen; Helle Merete Bhatia
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)