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Hepatocellular Defence Against Acidosis Is Preserved After Cold Storage by Helbling; Renner is a Medicine article available to read on EtoBox.

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## Background Primary non‐function of liver allografts is related to preservation time, during which hypoxia leads to intracellular accumulation of acid. Preservation‐induced failure of hepatocellular pH regulation may play a role in the pathogenesis of primary graft non‐function. ## Methods Using cultured/suspended rat hepatocytes and fluorimetric determination of intracellular pH, we determined whether preservation in University of Wisconsin solution (4°C) impairs hepatocellular defence mechanisms against acidosis. ## Results In non‐preserved, 24‐h‐preserved and 48‐h‐preserved hepatocytes acidified to pH 6.7–6.8, initial Na^+^/H^+^ antiport‐mediated H^+^ fluxes averaged 12 ± 5, 9 ± 5 and 12 ± 5 nmol μL^−1^ min^−1^ and initial Na^+^/HCO~3~^−^ symport‐mediated HCO~3~^−^ fluxes 7 ± 2, 7 ± 3 and 6 ± 2 nmol μL^−1^ min respectively (__P__ = NS). Preservation did not affect the inverse relationship between Na^+^/H^+^ antiport activity and intracellular pH. Thus, hepatocellular defence against intracellular acidosis is maintained during up to 48 h in University of Wisconsin solution. ## Conclusion Altered pH~i~ homeostasis is unlikely to play a role in the pathogenesis of primary non‐fun

Who reads Hepatocellular Defence Against Acidosis Is Preserved After Cold Storage?

Author

Helbling; Renner

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0014-2972)

Published

1998

Language

EN

Field

Medicine (Health Sciences)