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Can I read Unfolding and Refolding of Active Apple Polyphenol Oxidase on EtoBox?
Unfolding and Refolding of Active Apple Polyphenol Oxidase by Stéphane Mari; Laurence Marquès; Frédéric Breton; Yannis Karamanos; Jean-Jacques Macheix is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Unfolding and Refolding of Active Apple Polyphenol Oxidase about?
For the \_rst time\ unfolding "5 M guanidine# and refolding of partially proteolysed puri\_ed poly! phenol oxidase "PPOr# was achieved\ with 77) of activity recovered[ Optimal refolding conditions consisted in stepwise dialysis of guanidine treated extracts\ the dialysis bu}ers containing 0 M "NH 3 # 1 SO 3 and 099 mM CuSO 3 [ However\ CuSO 3 had limited e}ect on the recovering of PPOr activity\ whereas "NH 3 # 1 SO 3 was essential[ Concerning the PPO tertiary structure\ denaturing conditions "combinations of boiling and reducing agent# used on SDS!PAGE have shown "i# a compact tertiary structure and "ii# the presence of disul\_de bonds in PPOr\ accounting for the shift between 16 and 30 kDa\ and 30 and 31 kDa\ respectively[ Resistance to proteolytic cleavage was used to study the conformational changes induced by the denaturing treatments[ Folded PPOr was resistant to further proteolysis whereas unfolded PPO was totally digested\ indicating the role of tertiary structure of PPOr in the resistance to proteases[ Þ 0887 Elsevier Science Ltd[ All rights reserved
Who reads Unfolding and Refolding of Active Apple Polyphenol Oxidase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Stéphane Mari; Laurence Marquès; Frédéric Breton; Yannis Karamanos; Jean-Jacques Macheix
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)