Opening book details…
Can I read Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration on EtoBox?
Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration by Lu, Chenze (author);Saint-Pierre, Christine (author);Gasparutto, Didier (author);Roupioz, Yoann (author);Ravelet, Corinne (author);Peyrin, Eric (author);Buhot, Arnaud (author) is a Engineering article available to read on EtoBox.
What is Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration about?
Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of adenosine. Aptamers, short oligonucleotides, are known to be particularly efficient probes compared to antibodies thanks to their advantageous probe/target size ratio. Aptachains are formed from dimers with dangling ends followed by the split-aptamer binding triggered by the presence of the target. The high melting temperature of the dimers served as a calibration for the detection/quantification of the target based on the height and/or temperature shift of the aptachain melting peak.
Who reads Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Lu, Chenze (author);Saint-Pierre, Christine (author);Gasparutto, Didier (author);Roupioz, Yoann (author);Ravelet, Corinne (author);Peyrin, Eric (author);Buhot, Arnaud (author)
- Publisher
- MDPI AG
- Published
- 2021
- Language
- EN
- Field
- Engineering (Physical Sciences)