Can I read Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP‐2 on EtoBox?

Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP‐2 by Takahito Yuasa; Hiroko Kataoka; Naoki Kinto; Masahiro Iwamoto; Motomi Enomoto‐Iwamoto; Shun‐ichiro Iemura; Naoto Ueno; Yasuaki Shibata; Hisashi Kurosawa; Akira Yamaguchi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP‐2 about?

## Abstract The roles of __Sonic hedgehog__ (__Shh__) and __Bone morphogenetic protein‐2__ (__Bmp‐2__) in osteoblast differentiation were investigated using in vitro cell systems. Recombinant amino‐terminal portion of SHH (rSHH‐N) dose dependently stimulated ALP activity in C3H10T1/2 and MC3T3‐E1 cells. rSHH‐N induced expression of __Osteocalcin__ mRNA in C3H10T1/2 cells. A soluble form of the receptor for type IA BMP receptor antagonized rSHH‐N‐induced ALP activity in C3H10T1/2 and MC3T3‐E1 cells, indicating that BMPs are involved in SHH‐induced osteoblast differentiation. Simultaneous supplement with rSHH‐N and BMP‐2 synergistically induced ALP activity and expression of __Osteocalcin__ mRNA in C3H10T1/2 cells. Pretreatment with rSHH‐N for 6 h enhanced the response to BMP‐2 by increasing ALP activity in C3H10T1/2 and MC3T3‐E1 cells. Stimulatory effects of rSHH‐N and additive effects with rSHH‐N and BMP‐2 on ALP activity were also observed in mouse primary osteoblastic cells. Transplantation of BMP‐2 (1 μg) into muscle of mice induced formation of ectopic bone, whereas transplantation of r‐SHH‐N (1–5 μg) failed to generate it. These results indicate that __Shh__ plays important ro

Who reads Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP‐2?

Author

Takahito Yuasa; Hiroko Kataoka; Naoki Kinto; Masahiro Iwamoto; Motomi Enomoto‐Iwamoto; Shun‐ichiro Iemura; Naoto Ueno; Yasuaki Shibata; Hisashi Kurosawa; Akira Yamaguchi

Publisher

Wiley

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)