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Can I read Experimental treatment of colorectal cancer in mice with human T cells electroporated with NKG2D RNA CAR on EtoBox?
Experimental treatment of colorectal cancer in mice with human T cells electroporated with NKG2D RNA CAR by Zhendong Li; Zhixia Chi; Wei-Xia Ang; Can Chen; Johan CK Tay; Yu-Yang Ng; Xuehu Xu; Junjian Wang; Jianqing Zhu; Shu Wang is a Medicine article available to read on EtoBox.
What is Experimental treatment of colorectal cancer in mice with human T cells electroporated with NKG2D RNA CAR about?
Peritoneal metastasis is often present in end-stage neoplastic diseases, including recurrent colorectal cancer and is associated with decreased overall survival. Novel methods are needed. We constructed first-, second- and third-generation chimeric antigen receptors (CARs) specific for NKG2D ligands and modified human T cells with mRNA electroporation. NKG2D CAR expression was detectable for at least 6 days postelectroporation and mediated efficient cytotoxicity against NKG2DL+ tumor cells, but not NKG2DL-cells. Multiple infusions of the first-generation CAR-T cells into immunodeficient mice bearing established peritoneal colorectal xenografts led to significantly reduced tumor burden. mRNA CAR is an economical way to test new CARs and potentiates controlling on-target/off-tumor toxicity and cytokine storms. The use of NKG2D RNA CARs to treat colorectal peritoneal metastasis warrants further investigation.
Who reads Experimental treatment of colorectal cancer in mice with human T cells electroporated with NKG2D RNA CAR?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Zhendong Li; Zhixia Chi; Wei-Xia Ang; Can Chen; Johan CK Tay; Yu-Yang Ng; Xuehu Xu; Junjian Wang; Jianqing Zhu; Shu Wang
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)