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Production of Recombinant Plant Gum with Tobacco Cell Culture in Bioreactor and Gum Characterization by Jianfeng Xu; Elena Shpak; Tingyue Gu; Murray Moo‐Young; Marcia Kieliszewski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Many plant gums, such as gum arabic, contain hydroxyproline‐rich glycoproteins (HRGPs), which are also abundant components of the plant cell extracellular matrix. Here we expressed in transgenic BY2 __Nicotiana tabacum__ (tobacco) cells, a synthetic gene encoding a novel HRGP‐based gum, designated gum arabic‐8 or (GA)~8~. (GA)~8~ encoded eight repeats of the consensus polypeptide sequence of gum arabic glycoprotein (GAGP): Gly‐Pro‐His‐Ser‐Pro‐Pro‐Pro‐Pro‐Leu‐Ser‐Pro‐Ser‐Pro‐Thr‐Pro‐Thr‐Pro‐Pro‐Leu, in which most of the Pro residues were posttranslationally modified to hydroxyproline (Hyp). (GA)~8~ was expressed as a green fluorescent protein (GFP) fusion protein targeted to the culture medium, (GA)~8~GFP. The culture of the transgenic cells in a 5‐L bioreactor showed that the production of (GA)~8~GFP was cell growth‐associated. The extracellular yield of (GA)~8~GFP was 116.8 mg/L after 14 days of culture and accounted for 87% of the total fusion protein expressed. (GA)~8~GFP was purified from the culture medium by a combination of hydrophobic interaction, gel permeation, and reversed phase chromatography. Biochemical characterization indicated that the amino acid compos

Who reads Production of Recombinant Plant Gum with Tobacco Cell Culture in Bioreactor and Gum Characterization?

Author

Jianfeng Xu; Elena Shpak; Tingyue Gu; Murray Moo‐Young; Marcia Kieliszewski

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)