Can I read HP1γ function is required for male germ cell survival and spermatogenesis on EtoBox?

HP1γ function is required for male germ cell survival and spermatogenesis by Jeremy P Brown; Jörn Bullwinkel; Bettina Baron-Lühr; Mustafa Billur; Philipp Schneider; Heinz Winking; Prim B Singh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is HP1γ function is required for male germ cell survival and spermatogenesis about?

## Abstract ## Background HP1 proteins are conserved components of eukaryotic constitutive heterochromatin. In mammals, there are three genes that encode HP1-like proteins, termed HP1α, HP1β and HP1γ, which have a high degree of homology This paper describes for the first time, to our knowledge, the physiological function of HP1γ using a gene-targeted mouse. ## Results While targeting the __Cbx3__ gene (encoding the HP1γ protein) with a conditional targeting vector, we generated a hypomorphic allele (__Cbx3__ ^__hypo__^), which resulted in much reduced (barely detectable) levels of HP1γ protein. Homozygotes for the hypomorphic allele (__Cbx3__ ^__hypo__/__hypo__^) are rare, with only 1% of __Cbx3__ ^__hypo__/__hypo__^animals reaching adulthood. Adult males exhibit a severe hypogonadism that is associated with a loss of germ cells, with some seminiferous tubules retaining only the supporting Sertoli cells (Sertoli cell-only phenotype). The percentage of seminiferous tubules that are positive for L1 ORF1 protein (ORF1p) in __Cbx3__ ^__hypo__/__hypo__^testes is greater than that for wild-type testes, indicating that L1 retrotransposon silencing is reversed, leading to ectopic expressi

Who reads HP1γ function is required for male germ cell survival and spermatogenesis?

Author

Jeremy P Brown; Jörn Bullwinkel; Bettina Baron-Lühr; Mustafa Billur; Philipp Schneider; Heinz Winking; Prim B Singh

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central; Springer Science and Business Media LLC (ISSN 1756-8935)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)