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1) An approximately 5 kb genomic DNA fragment from Streptomyces aureofaciens was cloned into E. coli. 2) The recombinant E. coli accumulated polyhydroxybutyrate (PHB) inclusions in the cytoplasm when various carbon sources were used. 3) Analysis showed the accumulated polymer was isotactic homopolymer PHB with a molecular weight of 2.85x105. Recombinant E. coli accumulated 25-28 times more PHB than the native S. aureofaciens.

Author

hafsa

Language

EN