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A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection by N. Rahmah; B.H. Lim; A.Khairul Anuar; R.K. Shenoy; V. Kumaraswami; S.Lokman Hakim; P. Chotechuang; K. Kanjanopas; C.P. Ramachandran is a Medicine article available to read on EtoBox.

What is A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection about?

An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0,300, the IgG4 ELISA demonstrated specificity rates of 95.6-1 OO%, sensitivity rates of 96-lOO%, positive predictive values of 75 100% and negative predictive values of 98\*9-100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.

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Author

N. Rahmah; B.H. Lim; A.Khairul Anuar; R.K. Shenoy; V. Kumaraswami; S.Lokman Hakim; P. Chotechuang; K. Kanjanopas; C.P. Ramachandran

Publisher

Elsevier Science; Oxford University Press; Oxford University Press (OUP); ScienceOpen (ISSN 0035-9203)

Published

2001

Language

EN

Field

Medicine (Health Sciences)