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Dam‐dependent phase variation of Ag43 in Escherichia coli is altered in a seqA mutant by Jason Correnti; Vincent Munster; Teresa Chan; Marjan van der Woude is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary In __Escherichia coli__, phase variation of the outer membrane protein Ag43 encoded by the __agn43__ gene is mediated by DNA methylation and the global regu‐lator OxyR. Transcription of __agn43__ occurs (ON phase) when three Dam target sequences in the __agn43__ regulatory region are methylated, which prevents the repressor OxyR from binding. Conversely, transcription is repressed (OFF) when these Dam target sequences are unmethylated and OxyR binds. A change in expression phase requires a concomitant change in the DNA methylation state of these Dam target sequences. To gain insight into the process of inheritance of the expression phase and the DNA methylation state, protein–DNA interactions at __agn43__ were examined. Binding of OxyR at __agn43__ was sufficient to protect the three GATC sequences contained within its binding site from Dam‐dependent methylation __in vitro__, suggesting that no other factors are required to maintain the unmethylated state and OFF phase. To maintain the methylated state of the ON phase, however, Dam must access the hemimethylated __agn43__ region after DNA replication, and OxyR binding must not occur. OxyR bound hemimethylated __agn43__ D

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Author

Jason Correnti; Vincent Munster; Teresa Chan; Marjan van der Woude

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)