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The possibility of microarray‐based analysis using cell‐free placental mRNA in maternal plasma by Kiyonori Miura; Shoko Miura; Kentaro Yamasaki; Takako Shimada; Akira Kinoshita; Norio Niikawa; Koh‐ichiro Yoshiura; Hideaki Masuzaki is a Medicine article available to read on EtoBox.
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## Abstract ## Objective The purpose of this study is to investigate a possibility of overall assessment of cell‐free (CF) placental mRNAs in maternal plasma. ## Methods First, placenta‐predominantly expressed transcripts were selected by the analysis of GeneChip using three sets of placental tissues and corresponding maternal blood cells. Subsequently, a custom cDNA array panel of placenta‐predominantly expressed transcripts was designed and used to compare the RNA profiles of maternal plasma collected from 12 preeclamptic and 12 uncomplicated pregnancies. Scatter plots for the signal intensities of the comparative cDNA hybridization revealed either unchanged or aberrant patterns. ## Results We selected top 50 placenta‐predominantly expressed transcripts that were > 2500 times higher in placental tissues than in corresponding whole blood samples. A custom cDNA array analysis detected the aberrant pattern in five preeclamptic women with severe hypertension but not in seven preeclamptic women with mild hypertension (__P__ < 0.05, Fisher's direct method). The aberrant pattern of above RNA transcripts in maternal plasma was validated by quantitative real‐time reverse transcription‐pol
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- Author
- Kiyonori Miura; Shoko Miura; Kentaro Yamasaki; Takako Shimada; Akira Kinoshita; Norio Niikawa; Koh‐ichiro Yoshiura; Hideaki Masuzaki
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0197-3851)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)