Can I read ** In vitro ** inactivation of mammalian methionine synthase by nitric oxide on EtoBox?

** In vitro ** inactivation of mammalian methionine synthase by nitric oxide by A. NICOLAOU; S. H. KENYON; J. M. GIBBONS; T. AST; W. A. Gibbons is a Medicine article available to read on EtoBox.

What is ** In vitro ** inactivation of mammalian methionine synthase by nitric oxide about?

The research described here provides one mechanism of uniting current effects of nitric oxide (NO) with the elevated levels of homocysteine detected in patients with cardiovascular and other disease. Time‐ and dose‐dependent studies of the inhibition of purified mammalian methionine synthase by NO were performed. The __in vitro__ study gave an effective IC~50~ value of 3 μmol L^‐1^. Methionine synthase converts cellular homocysteine to methionine and is a major enzyme in the biosynthetic pathways for folates, __S__‐adenosylmethionine and biological methylations, sulphur amino acids and polyamines. Nitric oxide‐induced inactivation of methionine synthase alters the levels of these metabolites and could therefore provide a connection between the cardiovascular effects of NO, the plasma homo‐cysteine levels and cardiovascular diseases that is complementary to the more traditional NO‐induced stimulation of guanylate cyclase and the convertion of homocysteine to oxidized sulphur amino acids.

Who reads ** In vitro ** inactivation of mammalian methionine synthase by nitric oxide?

Author

A. NICOLAOU; S. H. KENYON; J. M. GIBBONS; T. AST; W. A. Gibbons

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0014-2972)

Published

1996

Language

EN

Field

Medicine (Health Sciences)