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Micromirror Total Internal Reflection Microscopy for High-performance Single Particle Tracking at Interfaces by Meng, Xuanhui; Sonn-Segev, Adar; Schumacher, Anne; Cole, Daniel; Young, Gavin; Thorpe, Stephen; Style, Robert W.; Dufresne, Eric R.; Kukura, Philipp is a scholarly article available to read on EtoBox.

What is Micromirror Total Internal Reflection Microscopy for High-performance Single Particle Tracking at Interfaces about?

Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterisation of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable when localisation precision and imaging speed are paramount due to the in principle infinite photon budget. Here, we show that micromirror-based total internal reflection dark field microscopy enables background suppression previously only reported for interferometric scattering microscopy, resulting in nm localisation precision at 6 $\mu$s exposure time for 20 nm gold nanoparticles with a 25 x 25 $\mu$m$^{2}$ field of view. We demonstrate the capabilities of our implementation by characterizing sub-nm deterministic flows of 20 nm gold nanoparticles at liquid-liquid interfaces. Our results approach the optimal combination of background suppression, localisation precision and temporal resolution achievable with pure scattering-based imaging and tracking of nanoparticles at regular interfaces.

Author

Meng, Xuanhui; Sonn-Segev, Adar; Schumacher, Anne; Cole, Daniel; Young, Gavin; Thorpe, Stephen; Style, Robert W.; Dufresne, Eric R.; Kukura, Philipp

Published

2021

Language

EN