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Phytohormone Regulation of Isoperoxidases in Catharanthus Roseus Suspension Cultures by Ferid Limam; Karim Chahed; Nedra Ouelhazi; Rachid Ghrir; Lazhar Ouelhazi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Key Word Index\*Catharanthus roseus^Apocynacea^cell suspension^phytohormones^per! oxidase isoforms^puri\_cation[ Abstract\*Peroxidase "POD# activity was investigated in Catharanthus roseus cell suspensions cultured under di}erent hormonal conditions[ Depletion of 1\3!dichlorophenoxyacetic acid "1\3!D# from the culture medium enhanced POD activity in cells and spent medium[ Addition of phytohormones\ in particular the auxin 1\3!D\ reduced POD activity in medium and cellular compartments and enhanced ionically cell!wall bound POD[ The di}erential modulation of POD is due to hormone e}ects on synthesis and:or accumulation of POD\ rather than on the secretion process[ Qualitative analysis showed that 1\3!D\ but not cytokinins\ regulated the synthesis of a basic isoform[ The cytokinin treatment seemed to a}ect acidic rather than basic isoforms[ The presence of basic POD is correlated with the capacity of cells to produce indole alkaloids[ The major extra! cellular basic isoperoxidase was puri\_ed to homogeneity from culture medium of Catharanthus roseus cell suspensions[ The isolated peroxidase is a haem protein with a M r of 22\999 and a pI close to 8[ The e}ect of pH on peroxidase act

Who reads Phytohormone Regulation of Isoperoxidases in Catharanthus Roseus Suspension Cultures?

Author

Ferid Limam; Karim Chahed; Nedra Ouelhazi; Rachid Ghrir; Lazhar Ouelhazi

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)