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Can I read The crystal structure of trypanothione reductase from the human pathogen Trypanosoma cruzi at 2.3 Å resolution: Trypanothione reductase structure on EtoBox?
The crystal structure of trypanothione reductase from the human pathogen Trypanosoma cruzi at 2.3 Å resolution: Trypanothione reductase structure by Yihong Zhang; Charles S. Bond; Susan Bailey; Mark L. Cunningham; Alan H. Fairlamb; William N. Hunter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The crystal structure of trypanothione reductase from the human pathogen Trypanosoma cruzi at 2.3 Å resolution: Trypanothione reductase structure about?
Trypanothione reductase (TR) is an NADPH-dependent flavoprotein unique to protozoan parasites from the genera Trypanosoma and Leishmania and is an important target for the design of improved trypanocidal drugs. We present details of the structure of TR from the human pathogen Trypanosoma cruzi, the agent responsible for Chagas' disease or South American trypanosomiasis. The structure has been solved by molecular replacement, using as the starting model the structure of the enzyme from the nonpathogenic Crithidia fasciculata, and refined to an R-factor of 18.9% for 53,868 reflections with F > or = sigma F between 8.0 and 2.3 A resolution. The model comprises two subunits (968 residues), two FAD prosthetic groups, two maleate ions, and 419 water molecules. The accuracy and geometry of the enzyme model is improved with respect to the C. fasciculata enzyme model. The new structure is described and specific features of the enzyme involved in substrate interactions are compared with previous models of TR and related glutathione reductases from human and Escherichia coli. Structural differences at the edge of the active sites suggest an explanation for the differing specificities toward g
Who reads The crystal structure of trypanothione reductase from the human pathogen Trypanosoma cruzi at 2.3 Å resolution: Trypanothione reductase structure?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yihong Zhang; Charles S. Bond; Susan Bailey; Mark L. Cunningham; Alan H. Fairlamb; William N. Hunter
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 1995
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)