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Synthesis of Cyclic Peptides and Peptide Libraries on a New Disulfide Linker by Werner Tegge; Wilfried Bautsch; Ronald Frank is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A new cysteine‐based disulfide linker for Fmoc solid phase peptide synthesis was developed (Fmoc‐Cys(3‐mercapto‐3‐methylbutanoic acid)OPp) that allows the on‐resin assembly and side chain deprotection of cyclic peptides. Model peptides and a cyclic peptide library of the structure [a‐a‐x‐x‐a‐a‐c] composed of D‐amino acids were assembled and the synthesis and cleavage conditions studied. The best cyclization results were obtained with PyBOP/HOAt/diisopropylethyl amine. Racemization rates of the cysteine in the analyzed model sequences were between 5.2 and 12.3%. Cleavage of the disulfide bond was best carried out with DTT in 50% 2‐propanol/100 mM ammonium bicarbonate. The cleaved peptides can be used directly in biological assays. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.

Who reads Synthesis of Cyclic Peptides and Peptide Libraries on a New Disulfide Linker?

Author

Werner Tegge; Wilfried Bautsch; Ronald Frank

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-2617)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)