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Reagent Preparation by Maltose Bioinnovations is a document available to read on EtoBox.
What is Reagent Preparation about?
The document describes a zymography protocol for detecting matrix metalloproteinase (MMP) activities in biological samples. Samples are subjected to SDS-PAGE in a resolving gel containing gelatin substrate. After electrophoresis, proteases re-nature and hydrolyze the gelatin, decreasing Coomassie staining at their position. Stained gels are scanned to detect and quantify MMP activity.
- Author
- Maltose Bioinnovations
- Language
- EN