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Induction of an AP endonuclease activity in Streptococcus mutans during growth at low pH by K. Hahn; R. C. Faustoferri; R. G. Quivey Jr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Induction of an AP endonuclease activity in Streptococcus mutans during growth at low pH about?

The oral microbe __Streptococcus mutans__ uses adaptive mechanisms to withstand the fluctuating pH levels in its natural environment. The regulation of protein synthesis is part of the mechanism of acid adaptation and tolerance in __S. mutans__. Here, we demonstrate that the organism's acid‐inducible protein repertoire includes an AP endonuclease activity. This abasic site‐specific endonuclease activity is present at greater levels in cells grown at low pH than in cells grown at pH 7, and is apparently independent of the RecA protein. Experiments using tetrahydrofuran or α‐deoxyadenosine‐containing substrates indicate that the activity induced at low pH may be similar to the activity of exonuclease III from __E. coli__. Acid‐adapted __S. mutans__ also shows an increased survival rate after exposure to near‐UV radiation in both the wild type and a __recA__ strain. Far‐UV radiation resistance is observed in the wild type only. The endonuclease activity was purified ≈500‐fold from an __S. mutans recA__ mutant strain grown at pH 5. Initial characterization revealed a 3′ to 5′ exonuclease activity, and showed additional functional similarities to DNA repair enzymes from other organisms.

Who reads Induction of an AP endonuclease activity in Streptococcus mutans during growth at low pH?

Author

K. Hahn; R. C. Faustoferri; R. G. Quivey Jr

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)