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Determination of Bestatin in Serum by High-performance Liquid Chromatography with Fluorescence Detection by Junichi Ishida; Masatoshi Yamaguchi; Masaaki Kai; Yosuke Ohkura; Masaru Nakamura is a Chemistry article available to read on EtoBox.

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A simple method for the determination of bestatin and its major metabolite in man, p-hydroxybestatin, in human serum was investigated; the method employs high-performance liquid chromatography with fluorescence detection. Bestatin and p-hydroxybestatin are oxidized to phenylacetaldehyde and p-hydroxyphenylacetaldehyde, respectively, with periodate, which are then converted into fluorescent compounds with 4,5-dimethoxy-1,2diaminobenzene. The compounds are separated by reversed-phase chromatography on LiChrosorb RP-18. The detection limits of bestatin and p-hydroxybestatin are 0.2 and 0.4 &ml serum, respectively. This method permits the precise determination of bestatin in serum (20 ~1) from patients administered beetatin. p-Hydroxybestatin in serum can not be measured by this method because of its low concentration (less than the detection limit).

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Author

Junichi Ishida; Masatoshi Yamaguchi; Masaaki Kai; Yosuke Ohkura; Masaru Nakamura

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1984

Language

EN

Field

Chemistry (Physical Sciences)