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Acid Invertase Is Predominantly Localized to Cell Walls of Both the Practically Symplasmically Isolated Sieve Element/companion Cell Complex and Parenchyma Cells in Developing Apple Fruits by D. P. Zhang; Y. M. Lu; Y. Z. Wang; C. Q. Duan; H. Y. Yan is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Abstract The acid invertase (__β__‐fructosidase, EC 3·2·1·26) was localized at subcellular level via immunogold electron microscopy in the phloem‐unloading zone of developing apple fruit. The enzyme (immunogold particles) was found to reside predominantly in the cell walls of the sieve element/companion cell (SE/CC) complex, phloem parenchyma cells and other parenchyma cells. There was almost no gold particle found in cytoplasm and vacuole. This distribution pattern remained unchanged throughout the growing season, but the enzyme numbers varied. The density of immunogold particles increased during fruit development. The immunoblotting of soluble and insoluble acid invertases provided a supporting proof for the assays of immunolocalization. The biochemical analysis showed a predominantly cell‐wall‐distributed activity of acid invertase that corresponds essentially with its amount distribution. The ultrastructural observations showed that there were numerous plasmodesmata between the parenchyma cells, but almost no plasmodesmium between the SE/CC complex and its surrounding parenchyma cells, practically resulting in the symplasmic isolation of the SE/CC complex. It is therefore su

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Author

D. P. Zhang; Y. M. Lu; Y. Z. Wang; C. Q. Duan; H. Y. Yan

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0140-7791)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)