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Dopamine‐induced inhibition of Na^+^‐K^+^‐ATPase activity requires integrity of actin cytoskeleton in opossum kidney cells by P. GOMES; P. SOARES-DA-SILVA is a Medicine article available to read on EtoBox.

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## ABSTRACT The present study evaluated the importance of the association between Na^+^‐K^+^‐ATPase and the actin cytoskeleton on dopamine‐induced inhibition of Na^+^‐K^+^‐ATPase activity. The approach used measures the transepithelial transport of Na^+^ in monolayers of opossum kidney (OK) cells, when the Na^+^ delivered to Na^+^‐K^+^‐ATPase was increased at the saturating level by amphotericin B. The maximal amphotericin B (1.0 __μ__g mL^–1^) induced increase in short‐circuit current (__I~sc~__) was prevented by ouabain (100 __μ__M) or removal of apical Na^+^. Dopamine (1 __μ__M) applied from the apical side significantly decreased (29 ± 5% reduction) the amphotericin B‐induced increase in __I~sc~__, this being prevented by the D~1~‐like receptor antagonist SKF 83566 (1 __μ__M) and the protein kinase C (PKC) inhibitor chelerythrine (1 __μ__M). Exposure of OK cells to cytochalasin B (1 __μ__M) or cytochalasin D (1 __μ__M), inhibitors of actin polymerization, from both cell sides reduced by 31 ± 4% and 36 ± 3% the amphotericin B‐induced increase in __I~sc~__ and abolished the inhibitory effect of apical dopamine (1 __μ__M), but not that of the PKC activator phorbol‐12,13‐dibutyrate

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Author

P. GOMES; P. SOARES-DA-SILVA

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0001-6772)

Published

2002

Language

EN

Field

Medicine (Life Sciences)