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Comparison of two methods of preparing enzyme-antibody conjugates: Application of these conjugates for enzyme immunoassay by Michael J. O'Sullivan; Ernesto Gnemmi; David Morris; Giorgio Chieregatti; Alan D. Simmonds; Madeleine Simmons; James W. Bridges; Vincent Marks is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Comparison of two methods of preparing enzyme-antibody conjugates: Application of these conjugates for enzyme immunoassay about?
Two methods of preparing enzyme-antibody conjugates were evaluated. High yields of conjugate were obtained with both methods. The first procedure utilizes the homobifunctional crosslinking reagent N,N'-o-phenylenedimaleimide. Sulfydryl residues were introduced into second antibodies by reaction with methyl-mercaptobutyrimidate. The modified antibodies were reacted with N&'-o-phenylenedimaleimide, excess reagent was removed by gel filtration, and the activated antibodies were cross-linked to /3-galactosidase. Up to 80% of the enzyme was conjugated to immunologically active antibody with approximately 90% retention of enzyme activity. The second method utilizes the heterobifunctionalmeta-maleimidobenzoyl-~-hydroxysuccinimide ester (MBS). The antibodies were reacted with MBS, excess reagent was removed by gel filtration and the activated antibodies were crosslinked to &galactosidase. Typically, approximately 80% of the enzyme was conjugated to immunologically active antibody with approximately 90% retention of both enzyme and antibody activity. Conjugates prepared using these two procedures were used as labels in an immunoassay system and were able to detect approximately 5 to 10 ng o
Who reads Comparison of two methods of preparing enzyme-antibody conjugates: Application of these conjugates for enzyme immunoassay?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Michael J. O'Sullivan; Ernesto Gnemmi; David Morris; Giorgio Chieregatti; Alan D. Simmonds; Madeleine Simmons; James W. Bridges; Vincent Marks
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1979
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)