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Corticospinal Neurons Exhibit a Novel Pattern of Cytoskeletal Gene Expression After Injury by S. A. Mikucki; Dr. M. M. Oblinger is a Neuroscience article available to read on EtoBox.

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We examined changes in the expression of major cytoskeletal protein mRNAs in adult hamster corticospinal neurons after axotomy. While a number of studies had determined that peripheral neurons exhibit major alterations in cytoskeletal gene expression after axotomy, no previous studies had addressed the question of whether or not intrinsic mammalian CNS neurons, which do not have the ability to successfully regenerate axons after injury, alter their expression of tubulin and neurofilament genes after injury. In the present study we used in situ hybridization methods to examine this issue. 35S-labeled cDNA probes for the low molecular weight neurofilament protein (NF-L) mRNA and an a-tubulin mRNA species (Mal) were used for in situ hybridizations of sections of the sensorimotor cortex obtained 2 , 7 , and 14 days after unilateral axotomy of the corticospinal tract in the caudal medulla. Film as well as emulsion autoradiography showed dramatic decreases in both a-tubulin and NF-L mRNA levels within axotomized neurons in layer Vb of the sensorimotor cortex. Tubulin mRNA levels were decreased as early as 2 days after injury whereas NF-L mRNA levels were not decreased until later times.

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Author

S. A. Mikucki; Dr. M. M. Oblinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1991

Language

EN

Field

Neuroscience (Life Sciences)