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Expression profiles of sex differentiation‐related genes during ontogenesis in the European sea bass acclimated to two different temperatures by Mercedes Blázquez; Laia Navarro‐Martín; Francesc Piferrer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The European sea bass is a teleost fish that lacks sex chromosomes and for which temperature influences sex ratios. However, correlation between temperature, developmental stage at a given age and sex‐specific gene expression is hampered by the lack of sex markers. To study this correlation, fish were exposed to feminizing (15°C) or masculinizing temperature (21°C) from 0–120 days post fertilization, throughout the thermosensitive period (TSP). Aromatase (__cyp19a1a__), 11β‐hydroxylase (__cyp11b__), androgen receptor (__arb__) and estrogen receptors (__era, erb1__ and __erb2__) were assessed by qPCR prior and during sex differentiation. Canonical discriminant analysis (CDA), with length—as proxy for developmental stage—and __cyp19a1a__ expression as predictors, was validated and used to reliably assign gonadal sex to fish sampled within and outside the TSP. Differences in __cyp19a1a__ and __cyp11b__ expression could be detected 1‐month before the first signs of histological sex differentiation. __Cyp19a1a__ and __cyp11b__ were significantly higher in future females and males, respectively, and revealed as robust molecular markers to predict future ovarian and testicular

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Author

Mercedes Blázquez; Laia Navarro‐Martín; Francesc Piferrer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1552-5007)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)