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Modified Granada Agar Medium for the detection of group B streptococcus carriage in pregnant women by G. Claeys; G. Verschraegen; M. Temmerman is a Medicine article available to read on EtoBox.

What is Modified Granada Agar Medium for the detection of group B streptococcus carriage in pregnant women about?

## Objectives: To improve the detection rate of group b streptococci (gbs) in pregnant women, aiming at the prevention of early-onset septicemia in the newborn. ## Methods: The yield from culturing two sites, vaginal and anorectal, on a modified granada medium (mgm) was compared with our standard approach of culturing a vaginal swab on blood agar (ba). ## Results: Samples were processed from 430 consecutive pregnant women. gbs was isolated from the vagina in 11.6% with ba, and in 13.7% with mgm. in 17.0% of anorectal samples, gbs was identified with mgm. the combination of both sites and media had a yield of 20.0%. mgm identified all but six (2%) of 310 gbs strains after aerobic incubation, with use of a cover slide, and missed only three strains (1%) after anaerobic incubation. ## Conclusions: Separate culture of vaginal and anorectal samples using the same mgm agar plate resulted in an increase in detection rate for gbs of 76% as compared to ba alone. the technique is simple and results are available after overnight incubation. mgm was confirmed as a specific medium for the identification of gbs, with a sensitivity of 98-99%.

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Author

G. Claeys; G. Verschraegen; M. Temmerman

Publisher

John Wiley and Sons; Elsevier ; Elsevier Limited; Elsevier BV; Test accounts (ISSN 1198-743X)

Published

2001

Language

EN

Field

Medicine (Health Sciences)