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Species Identification of Campylobacter jejuni and Campylobacter coli Isolates from Raw Poultry Products by MALDI–TOF MS and rRNA Sequence Analysis by Sulaiman, Irshad M; Hsieh, Ying-Hsin; Simpson, Steven is a Chemistry article available to read on EtoBox.

What is Species Identification of Campylobacter jejuni and Campylobacter coli Isolates from Raw Poultry Products by MALDI–TOF MS and rRNA Sequence Analysis about?

## Background: Campylobacter is a genus of gram-negative bacteria. it is considered one of the leading cause of diarrheal illness worldwide. incidence of human campylobacteriosis has increased significantly during recent decades; the increase has been directly linked with the advancement made in campylobacter detection methods. c. jejuni and c. coli are the two major human-pathogenic species that can colonize poultry and cause foodborne illness and outbreak. ## Objective: This study was carried out to rapidly identify campylobacter-like isolates recovered from raw poultry products by matrix-assisted laser desorption ionization (maldi)-time-of-flight (tof) ms and dna sequencing of 16s and 23s ribosomal rna (rrna) regions. ## Methods: Twenty-seven isolates of campylobacter-like organisms were isolated from raw poultry products and cultured on blood agars for maldi-tof ms analysis and dna isolation. for each isolate, one to two colonies were directly spotted on the vitek ms analysis. genomic dna was extracted from overnight bacterial culture. afterward, two-directional sanger sequencing of rrna gene was performed to confirm species identification on an abi 3500xl genetic analyzer. ##

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Author

Sulaiman, Irshad M; Hsieh, Ying-Hsin; Simpson, Steven

Publisher

Association of Official Analytical Chemists; The Journal of AOAC International; AOAC International; Oxford University Press (OUP) (ISSN 1060-3271)

Published

2019

Language

EN

Field

Chemistry (Physical Sciences)