Can I read Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse on EtoBox?

Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse by Ebrahim; Evans is a Medicine article available to read on EtoBox.

What is Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse about?

T16 mice contain a human 3′ untranslated sequence of the Thy 1.1 gene. Unlike normal mice they express Thy 1.1 protein on the podocytes which was immuno‐localized to podocyte apical and basal plasma membranes and filtration slit. When monoclonal anti‐Thy 1.1 antibody (OX7) was injected in nonproteinuric heterozygous mice there was rapid podocyte foot process swelling and proteinuria. Immunofluorescence showed granular glomerular OX7 binding at one hour. Progressive loss of pedicels occurred with 17.9 ± 2.5, 14.4 ± 1.1 and 10.5 ± 3.5 per 10 nm glomerular basement membrane (GBM) remaining 1, 6 and 24 hours, respectively, after 1 mg OX7, __vs__ 32.2 ± 2.0 in T16 mice given saline. Twenty‐four hour proteinuria was OX7 dose‐dependent, peaked at 1–3 days and reduced to near basal levels 9–11 days thereafter. Proteinuria was nonselective except at very low doses (0.1 mg OX7) where microalbuminuria was seen. F(ab′)~2~ OX7 administration also caused proteinuria in T16 mice. One milligram F(ab′)~1~ OX7 caused diffuse foot process swelling without manifest proteinuria in T16 mice. Anti‐Thy 1.1 IgM monoclonal antibody did not produce the effects of OX7 in T16 mice. Foot process swelling was no

Who reads Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse?

Author

Ebrahim; Evans

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0959-9673)

Published

1999

Language

EN

Field

Medicine (Life Sciences)