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Cu(I)–Glutathione complex: A potential source of superoxide radicals generation by Hernán Speisky; Maritza Gómez; Catalina Carrasco-Pozo; Edgar Pastene; Camilo Lopez-Alarcón; Claudio Olea-Azar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Cu(I)–Glutathione complex: A potential source of superoxide radicals generation about?

a b s t r a c t Cu 2+ ions and GSH molecules interact to swiftly form the complex Cu(I)-glutathione. We investigated the potential capacity of such complex to reduce molecular oxygen. The addition of SOD to a solution containing Cu(I)-glutathione led to a sustained decline of the basal oxygen level. Such effect was partially reverted by the addition of catalase. The complex was able to induce the reduction of cytochrome c and the oxidation of dyhydroethidium into 2-hydroxyethidium. Both effects were totally blocked by SOD. The ability of the complex to generate superoxide radicals was confirmed by EPR spin-trapping. Cu(I)-glutathione induce no oxidation of fluorescein, a hydroxyl radical-sensitive probe. We conclude that in solutions containing the complex, oxygen is continually reduced into superoxide, and that-in absence of interceptors-the latter radicals are quantitatively re-oxidized into molecular oxygen. We suggest that by functioning as a continuous source of superoxide, the complex could potentially affect a broad range of susceptible biological targets.

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Author

Hernán Speisky; Maritza Gómez; Catalina Carrasco-Pozo; Edgar Pastene; Camilo Lopez-Alarcón; Claudio Olea-Azar

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0968-0896)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)