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Can I read Sterile α-Motif Domain Requirement for Cellular Signaling and Survival on EtoBox?
Sterile α-Motif Domain Requirement for Cellular Signaling and Survival by Suhita Ray; Linda Chee; Daniel R. Matson; Nick Y. Palermo; Emery H. Bresnick; Kyle J. Hewitt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sterile α-Motif Domain Requirement for Cellular Signaling and Survival about?
Hundreds of sterile α-motif (SAM) domains have predicted structural similarities and are reported to bind proteins, lipids, or RNAs. However, the majority of these domains have not been analyzed functionally. Previously, we demonstrated that a SAM domain-containing protein, SAMD14, promotes SCF/proto-oncogene c-Kit (c-Kit) signaling, erythroid progenitor function, and erythrocyte regeneration. Deletion of a Samd14 enhancer (Samd14–Enh), occupied by GATA2 and SCL/TAL1 transcription factors, reduces SAMD14 expression in bone marrow and spleen and is lethal in a hemolytic anemia mouse model. To rigorously establish whether Samd14–Enh deletion reduces anemia-dependent c-Kit signaling by lowering SAMD14 levels, we developed a genetic rescue assay in murine Samd14–Enh^−/−^ primary erythroid precursor cells. SAMD14 expression at endogenous levels rescued c-Kit signaling. The conserved SAM domain was required for SAMD14 to increase colony-forming activity, c-Kit signaling, and progenitor survival. To elucidate the molecular determinants of SAM domain function in SAMD14, we substituted its SAM domain with distinct SAM domains predicted to be structurally similar. The chimeras were less effe
Who reads Sterile α-Motif Domain Requirement for Cellular Signaling and Survival?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Suhita Ray; Linda Chee; Daniel R. Matson; Nick Y. Palermo; Emery H. Bresnick; Kyle J. Hewitt
- Publisher
- The American Society for Biochemistry and Molecular Biology; American Society for Biochemistry and Molecular Biology; American Society for Biochemistry and Molecular Biology Inc.; American Society for Biochemistry & Molecular Biology (ASBMB); Elsevier BV (ISSN 0021-9258)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)