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Bovine mitochondrial DNA polymorphism in restriction endonuclease cleavage patterns and the location of the polymorphic sites by Tomomasa Watanabe; Yukimasa Hayashi; Reiji Semba; Nobuaki Ogasawara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Bovine mitochondrial DNA polymorphism in restriction endonuclease cleavage patterns and the location of the polymorphic sites about?

Cleavage patterns of mitochondrial DNA (mtDNA) by restriction endonuclease analysis were examined in four Japanese Black cows, three Japanese Shorthorn cows, and six Holstein cows. Seventeen restriction enzymes which recognize six base pairs and two restriction enzymes which recognize four base pairs were used in this study. Polymorphism was observed with three restriction enzymes, HindIII, TaqI, and MspI, and was detected within the breeds. Nucleotide substitution was determined in the HindIII polymorphic site by DNA cloning and sequencing; this is C----T at position 10126 of the URF-3 region. Furthermore, the MspI and TaqI polymorphic sites were located on the physical map.

Who reads Bovine mitochondrial DNA polymorphism in restriction endonuclease cleavage patterns and the location of the polymorphic sites?

Author

Tomomasa Watanabe; Yukimasa Hayashi; Reiji Semba; Nobuaki Ogasawara

Publisher

Springer; Springer-Verlag; Kluwer Academic/Plenum Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0006-2928)

Published

1985

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)