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Apoptosis and Mitosis in Gonocytes of the Rat Testis During Foetal and Neonatal Development by Barbara Boulogne; Robert Olaso; Christine Levacher; Philippe Durand; René Habert is a Medicine article available to read on EtoBox.

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This study was undertaken to determine the extent of apoptosis and mitosis in the various testicular cell types throughout rat development from foetal day 14.5 to postnatal days 9–10. Apoptotic activity was studied by detecting DNA fragmentation (TUNEL method) __in situ__. A TUNEL‐positive reaction was detected in gonocytes, while none of the other testicular cells were labelled. The morphology of the TUNEL‐positive gonocytes was characteristic of apoptotic cells and was different from that observed in experimentally induced necrosis. The percentage of stained gonocytes peaked on day 15.5–16.5 post‐conception (dpc), decreased thereafter and no TUNEL‐positive gonocytes were found from foetal day 18.5 onwards. On postnatal day 2, apoptosis resumed and increased to reach a maximum on day 7. Mitosis in the gonocytes, as evaluated by the immunodetection of 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation, was present during the same developmental periods but the ratio of BrdU‐positive/TUNEL‐positive gonocytes was much greater in the foetal period than in the neonatal period. In an organotypic culture system, the changes in the apoptotic and mitotic activities of the gonocytes in testicular

Who reads Apoptosis and Mitosis in Gonocytes of the Rat Testis During Foetal and Neonatal Development?

Author

Barbara Boulogne; Robert Olaso; Christine Levacher; Philippe Durand; René Habert

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0105-6263)

Published

1999

Language

EN

Field

Medicine (Health Sciences)