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Can I read RT-qPCR reveals opsin gene upregulation associated with age and sex in guppies ( Poecilia reticulata ) - a species with color-based sexual selection and 11 visual-opsin genes on EtoBox?
RT-qPCR reveals opsin gene upregulation associated with age and sex in guppies ( Poecilia reticulata ) - a species with color-based sexual selection and 11 visual-opsin genes by Christopher RJ Laver; John S Taylor is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is RT-qPCR reveals opsin gene upregulation associated with age and sex in guppies ( Poecilia reticulata ) - a species with color-based sexual selection and 11 visual-opsin genes about?
## Background PCR-based surveys have shown that guppies (__Poecilia reticulata__) have an unusually large visual-opsin gene repertoire. This has led to speculation that opsin duplication and divergence has enhanced the evolution of elaborate male coloration because it improves spectral sensitivity and/or discrimination in females. However, this conjecture on evolutionary connections between opsin repertoire, vision, mate choice, and male coloration was generated with little data on gene expression. Here, we used RT-qPCR to survey visual-opsin gene expression in the eyes of males, females, and juveniles in order to further understand color-based sexual selection from the perspective of the visual system. ## Results Juvenile and adult (male and female) guppies express 10 visual opsins at varying levels in the eye. Two opsin genes in juveniles, __SWS2B__ and __RH2-2__, accounted for >85% of all visual-opsin transcripts in the eye, excluding __RH1__. This relative abundance (RA) value dropped to about 65% in adults, as __LWS-A180__ expression increased from approximately 3% to 20% RA. The juvenile-to-female transition also showed __LWS-S180__ upregulation from about 1.5% to 7% RA. Fina
Who reads RT-qPCR reveals opsin gene upregulation associated with age and sex in guppies ( Poecilia reticulata ) - a species with color-based sexual selection and 11 visual-opsin genes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Christopher RJ Laver; John S Taylor
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1471-2148)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)