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Can I read Mitigating Clonal Variation in Recombinant Mammalian Cell Lines on EtoBox?
Mitigating Clonal Variation in Recombinant Mammalian Cell Lines by Jae Seong Lee; Helene Faustrup Kildegaard; Nathan E. Lewis; Gyun Min Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mitigating Clonal Variation in Recombinant Mammalian Cell Lines about?
Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. We propose a new approach for a next-generation cell line development platform that can minimize clonal variation. Combined with the knowledge-based selection of ideal integration sites and engineering targets, targeted integration-based cell line development will allow tailored control of recombinant gene expression with predicted phenotypes.
Who reads Mitigating Clonal Variation in Recombinant Mammalian Cell Lines?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jae Seong Lee; Helene Faustrup Kildegaard; Nathan E. Lewis; Gyun Min Lee
- Publisher
- Elsevier BV
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)