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Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood–brain barrier in a mice hemorrhage model by Anatol Manaenko; Hank Chen; Jerome Kammer; John H. Zhang; Jiping Tang is a Neuroscience article available to read on EtoBox.
What is Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood–brain barrier in a mice hemorrhage model about?
Aims: Intracerebral hemorrhage is one of the most devastating subtypes of stroke, leaving survivors with severe neurological deficits. Disruption of the blood brain barrier (BBB) following hemorrhage results in the development of vasogenic brain edema, a most life-threatening event after such events as intracerebral hemorrhage (ICH). The Evans Blue assay is a popular method for the quantification of BBB disruption. Although this method is in common use, there are several protocols of the assay in the literature which vary in the route of administration, as well as the circulation time of the stain. In this study, we compared the amounts of accumulated stain in brain tissue following intraperitoneal versus intravenous injection at 0.5, 3 and 24 h of circulation time. Methods: 58 CD-1 mice were used. Animals were divided into ICH (N = 42), sham groups (N = 6) and naïve (N = 10). ICH animals received stereotactic injection of collagenase type VII into the right basal ganglia. Sham animals received only needle trauma. Evans Blue stain was injected 24 h after collagenase injection or needle trauma. The consistency of ICH produced was characterized by estimation of hematoma volume via he
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- Author
- Anatol Manaenko; Hank Chen; Jerome Kammer; John H. Zhang; Jiping Tang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)
- Published
- 2011
- Language
- EN
- Field
- Neuroscience (Life Sciences)