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In Vitro Toxicity Testing of Zinc Tetrasulfophthalocyanines in Fibroblast and Keratinocyte Cells for the Treatment of Melanoma Cancer by Photodynamic Therapy by K. Maduray; A. Karsten; B. Odhav; T. Nyokong is a Medicine article available to read on EtoBox.

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A series of water-soluble tetrasulfonated metallophthalocyanines (MPcs) dyes have been studied to be used as a drug or photosensitizer (PS) in photodynamic therapy (PDT) for the treatment of cancers. During PDT the PS is administrated intravenously or topically to the patient before laser light at an appropriate wavelength is applied to the cancerous area to activate the PS. The activated PS will react with oxygen typically present in the cancerous tissue to generate reactive oxygen species for the destruction of the cancerous tissue. This in vitro study aimed at investigating the cytotoxic effects of different concentrations of zinc tetrasulfophthalocyanines (ZnTSPc) activated with a diode laser (k = 672 nm) on melanoma, keratinocyte and fibroblast cells. To perform this study 3 Â 10 4 cells/ml were seeded in 24-well plates and allowed to attach overnight, after which cells were treated with different concentrations of ZnTSPc. After 2 h, cells were irradiated with a constant light dose of 4.5 J/cm 2 . Post-irradiated cells were incubated for 24 h before cell viability was measured using the CellTiter-Blue Viability Assay. Data indicated high concentrations of ZnTSPc (60-100 lg/ml)

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Author

K. Maduray; A. Karsten; B. Odhav; T. Nyokong

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1011-1344)

Published

2011

Language

EN

Field

Medicine (Physical Sciences)