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Can I read iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model on EtoBox?
iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model by Hurst, José; MuellerâBuehl, Ana Maria; Hofmann, Lisa; Kuehn, Sandra; Herms, Fenja; Schnichels, Sven; Joachim, Stephanie Christine is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model about?
Nitrite oxide plays an important role in the pathogenesis of various retinal diseases, especially when hypoxic processes are involved. This degeneration can be simulated by incubating porcine retinal explants with CoCl . Here, the therapeutic potential of iNOS-inhibitor 1400W was evaluated. Degeneration through CoCl and treatment with the 1400W were applied simultaneously to porcine retinae explants. Three groups were compared: control, CoCl , and CoCl + iNOS-inhibitor (1400W). At days 4 and 8, retinal ganglion cells (RGCs), bipolar, and amacrine cells were analysed. Furthermore, the influence on the glia cells and different stress markers were evaluated. Treatment with CoCl resulted in a significant loss of RGCs already after 4 days, which was counteracted by the iNOS-inhibitor. Expression of HIF-1α and its downstream targets confirmed the effective treatment with 1400W. After 8 days, the CoCl group displayed a significant loss in amacrine cells and also a drastic reduction in bipolar cells was observed, which was prevented by 1400W. The decrease in microglia could not be prevented by the inhibitor. CoCl induces strong degeneration in porcine retinae by mimicking hypoxia, damagin
Who reads iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hurst, José; MuellerâBuehl, Ana Maria; Hofmann, Lisa; Kuehn, Sandra; Herms, Fenja; Schnichels, Sven; Joachim, Stephanie Christine
- Publisher
- Carol Davila University of Medicine Bucharest, Romania; Wiley (Blackwell Publishing); Wiley-Blackwell; Wiley (ISSN 1582-1838)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)