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In Vitro Capacitation and the Chemically Induced Acrosome Reaction in Bovine Spermatozoa by William Byrd is a Environmental Science article available to read on EtoBox.
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## Abstract The effect of bovine serum albumin (BSA), calcium, and ionophore A23187 on in vitro capacitation and the induction of the acrosome reaction in bovine spermatozoa was examined using light, fluorescent, and electron microscopy and spectrofluorometry. Transmission electron microscopy of fixed spermatozoa labeled with the plant lectin concanavalin A indicated that a significant redistribution of lectin binding sites requires at least three hours of incubation in a capacitating medium that contains BSA and calcium. Spermatozoa underwent capacitation and the acrosome reaction following a minimum incubation time of 2–3 hours in capacitating medium in the continuous presence of calcium ionophore A23187 (1–10 μm). The induction of the acrosome reaction was determined by light microscopy of fixed‐stained cells as well as transmission electron microscopy. These data suggest that a minimum of 2–3 hours is required for in vitro capacitation of bovine spermatozoa. Capacitation was also examined using the tetracycline‐HCl (T‐HCl) binding assay of Ericsson ('67). Measurement of fluorescence by spectrofluorometry and by fluorescence microscopy demonstrated that the level of fluorescent
Who reads In Vitro Capacitation and the Chemically Induced Acrosome Reaction in Bovine Spermatozoa?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- William Byrd
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)
- Published
- 1981
- Language
- EN
- ISBN
- 9781402150166
- Field
- Environmental Science (Life Sciences)