Can I read Combined analysis of T cell receptor γ and immunoglobulin heavy chain gene rearrangements at the single-cell level in lymphomas with dual genotype on EtoBox?

Combined analysis of T cell receptor γ and immunoglobulin heavy chain gene rearrangements at the single-cell level in lymphomas with dual genotype by B. Vergier; P. Dubus; A. Kutschmar; M. Parrens; J. Ferrer; A. de Mascarel; J. P. Merlio is a Medicine article available to read on EtoBox.

What is Combined analysis of T cell receptor γ and immunoglobulin heavy chain gene rearrangements at the single-cell level in lymphomas with dual genotype about?

## Abstract By prospectively studying immunoglobulin heavy chain gene (__IgH)__ and T cell receptor gamma (__TCR__γ) gene rearrangements in 398 lymphoma cases, a dual genotype was observed in 13% of B cell and 11% of T cell lymphomas. According to histological subtype, the highest incidence was observed for mantle cell lymphomas (32%) and lymphoplasmacytic lymphoma (21%) among B cell lymphomas, and for angioimmunoblastic lymphoma (AILT) (46%) and Sézary syndrome (SS) (50%) among T cell lymphomas. To determine whether the dual genotype corresponds to the presence of two distinct monoclonal populations or to the presence of both rearrangements within the same lymphoma cells, single‐cell microdissection was used after immunohistochemistry and a single‐cell combined __IgH__ and __TCR__γ gene analysis was designed after a whole‐genome amplification step. This protocol was applied to the study of two nodal B cell lymphomas (one diffuse large B cell lymphoma and one mantle cell lymphoma) and two cutaneous T cell lymphomas (one AILT and one SS). Two cases (SS and mantle cell lymphoma) were true bigenotypic lymphomas, as both __IgH__ and __TCR__γ monoclonal rearrangements were detected in t

Who reads Combined analysis of T cell receptor γ and immunoglobulin heavy chain gene rearrangements at the single-cell level in lymphomas with dual genotype?

Author

B. Vergier; P. Dubus; A. Kutschmar; M. Parrens; J. Ferrer; A. de Mascarel; J. P. Merlio

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2002

Language

EN

Field

Medicine (Health Sciences)