Can I read Heterogeneity of mesothelioma cell lines as defined by altered genomic structure and expression of the NF2 gene on EtoBox?

Heterogeneity of mesothelioma cell lines as defined by altered genomic structure and expression of the NF2 gene by Brigitte Deguen; Laurence Goutebroze; Marco Giovannini; Cécile Boisson; Ronald van der Neut; Marie-Claude Jaurand; Gilles Thomas is a Medicine article available to read on EtoBox.

What is Heterogeneity of mesothelioma cell lines as defined by altered genomic structure and expression of the NF2 gene about?

Germ-line mutations in the neurofibromatosis 2 (NF2) gene cause a susceptibility to the development of schwannoma and meningioma, 2 mostly benign tumors of neural crest origin. Bi-allelic inactivation of this gene has been observed in sporadic schwannomas and meningiomas. The NF2 gene may also be somatically inactivated in human malignant mesotheliomas (HMMs). Surprisingly, patients with an NF2 germ-line mutation have not been reported to be at an increased risk for this highly invasive tumor of mesodermal origin. To investigate in HMMs the silencing mechanism of the NF2 gene, we have analyzed its structure and expression in a series of 18 cell lines derived from HMMs. NF2 gene alterations were identified at a genomic level in 7 cell lines and were associated with a marked decrease in the concentration of the NF2 transcript. This decrease was also observed in 4 additional cell lines with no identified NF2 mutation. The 11 cell lines presented evidence suggesting deletion of one NF2 allele. None of these enabled the detection of normal or truncated forms of the NF2 protein by immunoprecipitation/ immunoblot analyses. In the 7 remaining cell lines, NF2 mRNA and NF2 protein were easil

Who reads Heterogeneity of mesothelioma cell lines as defined by altered genomic structure and expression of the NF2 gene?

Author

Brigitte Deguen; Laurence Goutebroze; Marco Giovannini; Cécile Boisson; Ronald van der Neut; Marie-Claude Jaurand; Gilles Thomas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1998

Language

EN

Field

Medicine (Health Sciences)