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Can I read Evaluation of the phosphorescent palladium(II)–coproporphyrin labels in separation-free hybridization assays on EtoBox?
Evaluation of the phosphorescent palladium(II)–coproporphyrin labels in separation-free hybridization assays by Martina Burke; Paul J O’Sullivan; Aleksi E Soini; Helen Berney; Dmitri B Papkovsky is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Evaluation of the phosphorescent palladium(II)–coproporphyrin labels in separation-free hybridization assays about?
Palladium(II)-coproporphyrin label and a set of corresponding monofunctional labeling reagents with different linker arms were evaluated for labeling of oligonucleotides and subsequent use in hybridization assays. The properties of resulting oligonucleotide probes including phosphorescence spectra, quantum yields, lifetimes, and labeling yields were examined as functions of the label and oligonucleotide structures. Upon hybridization with complementary sequences bearing dabcyl, QSY-7, and rhodamine green dyes, the probes displayed strong quenching due to close proximity effects. Intensity and lifetime changes of the phosphorescence, distance, and temperature dependences were investigated in detail. The potential of the new label and probes for sensitive and separation-free hybridization assays was discussed.
Who reads Evaluation of the phosphorescent palladium(II)–coproporphyrin labels in separation-free hybridization assays?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Martina Burke; Paul J O’Sullivan; Aleksi E Soini; Helen Berney; Dmitri B Papkovsky
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)