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Caspase-9-dependent Decrease of Nuclear Pore Channel Hydrophobicity Is Accompanied by Nuclear Envelope Leakiness by Armin Kramer; Ivan Liashkovich; Hans Oberleithner; Victor Shahin is a Materials Science article available to read on EtoBox.

What is Caspase-9-dependent Decrease of Nuclear Pore Channel Hydrophobicity Is Accompanied by Nuclear Envelope Leakiness about?

Advances in nanomedicine require conceptual understanding of physiological processes. Apoptosis is a fundamental physiological process that is characterized, among other things, by an increased permeability of the nuclear envelope (NE). The latter is a tight transport barrier, known to restrict nuclear delivery rate of therapeutic nanoparticles. Therefore, an understanding of the underlying mechanism that leads to the breakdown of the barrier during apoptosis could stimulate the development of new approaches in gene therapy. We set out to elucidate this mechanism following induction of apoptosis on isolated cell nuclei. We tested the hypothesis whether caspases, mediators of apoptosis, trigger the NE leakiness at the level of the nuclear pore complexes (NPCs) using fluorescence techniques. As the permeability barrier inside the NPC channel is thought to be based on hydrophobic-hydrophobic protein interactions we further investigated the NPC channel hydrophobicity using atomic force microscopy. Caspase-9 was found to induce NE leakiness to large macromolecules. Leakiness was prevented by pretreatment of NPCs with an importin-β mutant, which irreversibly binds and thereby obstructs t

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Author

Armin Kramer; Ivan Liashkovich; Hans Oberleithner; Victor Shahin

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1549-9634)

Published

2010

Language

EN

Field

Materials Science (Physical Sciences)