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Factors of the Human Embryo Culture System That May Affect Media Evaporation and Osmolality by E Mestres; M García-Jiménez; A Casals; J Cohen; M Acacio; A Villamar; Q Matia-Algué; G Calderón; N Costa-Borges is a Medicine article available to read on EtoBox.

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## Abstract ## STUDY QUESTION Which lab-related factors impact the culture system’s capacity to maintain a stable osmolality during human embryo culture? ## SUMMARY ANSWER Incubator humidity, the volume of mineral oil, the type of culture media and the design of time-lapse dishes have been identified as important parameters that can cause an impact on media evaporation and consequently osmolality during culture. ## WHAT IS KNOWN ALREADY Culture medium is a critical component in human embryo culture. Minimizing its evaporation during culture is an adequate strategy to stabilize osmolality and, as a result, improving culture conditions and clinical outcomes. ## STUDY DESIGN, SIZE, DURATION The studied variables included media composition and supplementation; volume of mineral oil; incubator humidification; and the type of dish and incubator used. Additionally, six time-lapse dish models were compared in their ability to prevent evaporation. ## PARTICIPANTS/MATERIALS, SETTING, METHODS Dishes were incubated in parallel to analyze osmolality during culture between groups: synthetic oviductal medium enriched with potassium versus human tubal fluid medium; protein versus no protein supple

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Author

E Mestres; M García-Jiménez; A Casals; J Cohen; M Acacio; A Villamar; Q Matia-Algué; G Calderón; N Costa-Borges

Publisher

Oxford University Press (OUP)

Published

2021

Language

EN

Field

Medicine (Health Sciences)