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This document describes an experiment to isolate and identify photosynthetic pigments in spinach leaves using chromatography and spectrophotometry. Students will extract pigments from spinach using chromatography paper to separate the pigments by polarity into distinct bands. They will measure the distance traveled by each pigment band to calculate Rf values for identification. The pigments will then be extracted and their absorption spectra measured using a spectrophotometer to identify the pigments based

Author

Yujin Seo

Language

EN