About this Engineering article
Hydrophobic Modification Improves the Delivery of Cell-penetrating Peptides to Eliminate Intracellular Pathogens in Animals by Qi Tang; Peng Tan; Zhaolai Dai; Tao Wang; Shenrui Xu; Yakun Ding; Junqi Jin; Xin Zhang; Yucheng Zhang; Chenlong Zhou; Zitian Yue; Huiyang Fu; Junshu Yan; Xi Ma is a Engineering article available to read on EtoBox.
Infections induced by intracellular pathogens are difficult to eradicate due to poor penetration of antimicrobials into cell membranes. It is of great importance to develop a new generation of antibacterial agents with dual functions of efficient cell penetration and bacterial inhibition. In this study, the association between hydrophobicity and cell-penetrating peptide delivery efficiency was investigated by fragment interception and hydrophobicity modification of natural porcine antimicrobial peptide PR-39 and the combination of cationic cell-penetrating peptide (R6) with antimicrobial peptide fragments modified with hydrophobic residues. The chimeric peptides P3I7 and P3L7, obtained through biofunctional screening, exhibited potent broad-spectrum antibacterial activity and low cytotoxicity. Moreover, P3I7 and P3L7 can effectively penetrate cells to eliminate intracellular pathogens mainly through endocytosis. The membrane destruction mechanism makes the peptides fast sterilizers and less prone to developing drug resistance. Finally, their good biocompatibility and antibacterial infection effects were verified in mice and piglets. To conclude, the chimeric peptides P3I7 and P3L7
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Qi Tang; Peng Tan; Zhaolai Dai; Tao Wang; Shenrui Xu; Yakun Ding; Junqi Jin; Xin Zhang; Yucheng Zhang; Chenlong Zhou; Zitian Yue; Huiyang Fu; Junshu Yan; Xi Ma
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)