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Detection of Epstein–Barr virus antigens and DNA in major and minor salivary glands using immunocytochemistry and polymerase chain reaction: Possible relationship with Sjogren's syndrome by E. M. Deacon; Dr J. B. Matthews; A. J. C. Potts; J. Hamburger; I. S. Bevan; L. S. Young is a Medicine article available to read on EtoBox.

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## Abstract This study has investigated the presence of Epstein–Barr virus (EBV) in parotid (__n__ = 12), submandibular (__n__ = 15), and minor salivary glands (__n__ = 25) using immunohistochemical methods for detection of EBV‐encoded antigens and the polymerase chain reaction (PCR) for detection of viral DNA. Major salivary glands were from patients without connective tissue disease. Labial glands were from patients with primary Sjogren's syndrome (__n__ = 10), rheumatoid arthritis (__n__ = 8), or from normal individuals (__n__ = 7). None of the glands exhibited specific reactivity for lytic (EA‐D, EA‐R, VCA) or latent (EBNA‐2, LMP) viral antigens. Antibodies to EA‐D, when used at 20–50 times their optimal concentration, gave lumenal staining of ducts and acini of all the specimens tested (__n__ = 14), irrespective of the presence (__n__ = 8) or absence (__n__ = 6) of EBV‐DNA by PCR. Ductal immunoreactivity for the EBV/C3d (CR2, CD21) receptor was found in 40 per cent of specimens. PCR detected EBV‐DNA in 64 per cent submandibular, 46 per cent parotid, and 80 per cent of minor glands. There were no significant differences in the detection of EBV‐DNA between specimen/patient group

Who reads Detection of Epstein–Barr virus antigens and DNA in major and minor salivary glands using immunocytochemistry and polymerase chain reaction: Possible relationship with Sjogren's syndrome?

Author

E. M. Deacon; Dr J. B. Matthews; A. J. C. Potts; J. Hamburger; I. S. Bevan; L. S. Young

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

1991

Language

EN

Field

Medicine (Health Sciences)