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Ischemia-Triggered Translocation and Inactivation of Protein Kinase C Isoforms in the Fetal Brain by Ephraim Yavin; Javier Inserte-Igual; Shosh Gil is a Neuroscience article available to read on EtoBox.

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Abstract: The kinetics of protein kinase C (PKC) translocation and down‐regulation in the 20‐day‐old fetal brain following short and long episodes of maternal‐fetal blood flow occlusion were examined. Restriction for up to 15 min increased the specific enzymatic activity in the membrane by 73%, indicative of translocation. After a 30‐min restriction and a 2.5‐h reperfusion the total PKC activity in the cytosol was reduced to ∼50%, consistent with down‐regulation/inactivation. The total membrane PKC activity remained unchanged. Several PKC isoenzymes, including α, β1, β2, ε, and ζ, but not γ, were identified in the fetal brain on western blots using specific antibodies. Compared with postnatal day 15, a greater proportion of the fetal PKC isoforms, particularly α and ε, were membrane bound. α, β2, ε, and ζ, but not β1, were translocated into the membrane compartment after episodes of ischemia alone or ischemia and reperfusion. There were no major identifiable proteolytic fragments in the 50‐kDa region. Major losses in the total enzymatic activity were encountered in both cytosol and membrane fractions after storage of the enzyme for 10 days at 4°C. These losses were less profound in

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Author

Ephraim Yavin; Javier Inserte-Igual; Shosh Gil

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1995

Language

EN

Field

Neuroscience (Life Sciences)