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Can I read Investigation of Liquid–solid Hydrodynamic Boundary Layers and Oxygen Requirements in Hairy Root Cultures on EtoBox?
Investigation of Liquid–solid Hydrodynamic Boundary Layers and Oxygen Requirements in Hairy Root Cultures by Gary R. C. Williams; Pauline M. Doran is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Investigation of Liquid–solid Hydrodynamic Boundary Layers and Oxygen Requirements in Hairy Root Cultures about?
Rates of oxygen uptake, growth and alkaloid production by hairy roots in submerged culture were investigated using a recirculation reactor allowing operation at high liquid velocities for removal of hydrodynamic boundary layers. Measurements were performed at dissolved oxygen tensions of 31-450% air saturation. Critical oxygen concentrations for Atropa belladonna hairy roots were above air saturation, viz. 100-125% air saturation for oxygen uptake and 150% air saturation for growth, demonstrating that these roots cultivated in reactors with air sparging are oxygen-limited. The critical oxygen tension for oxygen uptake by Solanum aviculare hairy roots was 75% air saturation. Both the specific oxygen uptake rate and specific growth rate of A. belladonna hairy roots were dependent on the mass (g dry weight) of roots present; even in the absence of boundary layers, growth did not remain exponential over the entire culture period. Cryo-scanning electron microscopy showed that hairy roots grown submerged in liquid medium were covered with thick layers of hydrated mucilage and root hairs, representing a significant additional barrier to oxygen transfer. Roots protruding out of the liquid
Who reads Investigation of Liquid–solid Hydrodynamic Boundary Layers and Oxygen Requirements in Hairy Root Cultures?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gary R. C. Williams; Pauline M. Doran
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)